Research

In vitro bioassay as a predictor of in vivo response

Ross Barnard¹ and Konstantin G Gurevich²

¹  Department of Biochemistry, The University of Queensland, Brisbane, Qld 4072, Australia

²  UNESCO Chair in healthy life for sustainable development, Moscow State University of Medicine and Dentistry, Delegatskay ulitsa, 20/1, 103473, Moscow, Russian Federation

author email corresponding author email

Theoretical Biology and Medical Modelling 2005, 2:3doi:10.1186/1742-4682-2-3

Published:	7 February 2005

Abstract

Background

There is a substantial discrepancy between in vitro and in vivo experiments. The purpose of the present work was development of a theoretical framework to enable improved prediction of in vivo response from in vitro bioassay results.

Results

For dose-response curve reaches a plateau in vitro we demonstrated that the in vivo response has only one maximum. For biphasic patterns of biological response in vitro both the bimodal and biphasic in vivo responses might be observed.

Conclusion

As the main result of this work we have demonstrated that in vivo responses might be predicted from dose-effect curves measured in vitro.