Figure 6.

PPF defect as a precursor to CDH in the rodent nitrofen model. (A) E13.5 transverse section of embryonic rat exposed to nitrofen, with a posterior defect (hollow arrow) in the left PPF (star). The section is through the mid-portion of the PPF. Lu, lung; VC, vena cava; E, esophagus. (B) Reconstruction is used to define the PPF defect in three dimensions. The upper image is of normal left and right PPFs with the perspective of looking through the left lateral cervical wall of an E13.5 rat. The lower image shows a left-sided defect in the PPF (hollow arrowhead). Scale bars = 100 μm. (Images reproduced with editorial permission from [11].)