Table 1

Kinetic parameters of Escherichia coli adenylosuccinate synthetase. Our model output, and data provided from the literature.
Parameter
 Model output
 Literature data
 Reference
KmGTP
 0.023 mM
 0.01 mM
 7


0.048 mM
 6


0.0221 mM
 10


0.0262 ± 0.0023 mM
 12
KmIMP
 0.02 mM
 0.02 mM
 7


0.054 mM
 6


0.0243 mM
 10


0.0278 ± 0.0013 mM
 12
KmASP
 0.3 mM
 0.35 mM
 7


0.225 ± 0.016 mM
 9


0.27 mM
 6


0.191 mM
 10


0.23 ± 0.04 mM
 12
KiSUCC*
 8 mM
 -
 -
KiGDP
 0.008 mM
 0.022 mM
 6
KiASUC
 0.0075 mM
 0.27 mM (for IMP)**
 6


0.47 mM (for ASP)
 6
KmMg
 0.08 mM
 0.114 ± 0.026 mM
 9
KiGMP
 0.024 mM
 0.092 mM (for GTP)
 6


0.074 mM (for IMP)
 6
KiAMP
 0.01 mM
 0.095 mM
 6

* The effect of SUCC was examined by Rudolph and Fromm [7]. However, their formula is too complicated (it describes the effect of one inhibitor using four different constants) and the inhibition constants were not evaluated.

** In 1963 Wyngaarden and Greenland [6] proposed that ASUC is a competitive inhibitor of both IMP and ASP, and GMP is a competitive inhibitor of both IMP and GTP.

Oshchepkova-Nedosekina and Likhoshvai Theoretical Biology and Medical Modelling 2007 4:11   doi:10.1186/1742-4682-4-11