Figure 5.

Calculated bonds for high Fit value sites in binding domain. A 3^rd-order component was observed between Lys-27, Gln-41, and Asp-52 which appears to help conform the beta strand/loop leading to the binding site. The bonded pair Leu-43 and Leu-50 help conform the beta strand and loop leading away from the binding site. These 5 sites were among the 7 highest Fit value sites for ubiquitin suggesting that they play a critical role in conforming the binding domain. (Bonds were computed using the software MacPyMOL)