|
Physiochemical parameters of EcoRII as predicted from the amino acid sequence alignments |
|
| Physicochemical parameter |
Value |
|
|
|
| Number of amino acids: |
404 |
| Molecular Weight |
45611 |
| Theoretical PI |
6.10 |
| Total number of negatively charged residues (Asp+Glu) |
52 |
| Total number of positive residues (Arg+Lys) |
43 |
| Atomic composition: |
|
| • Carbon(C) |
2053 |
| • Hydrogen(H) |
3205 |
| • Nitroge(N) |
565 |
| • Oxygen(O) |
393 |
| • Sulfur |
10 |
| Total number of atoms: |
6426 |
| Formula: |
C2053H3205N565O593S10 |
| Extinction coefficients: |
47120(46870) |
| Estimated half-life(hours) |
|
| • (mammalian reticulocytes, in vitro) |
30 hour |
| • (yeast, in vivo) |
> 20 hours |
| • (Escherichia coli, in vivo) |
> 10 hours |
| Instability index: |
45.04 |
| Aliphatic index: |
97.05 |
| Grand average of hydropathicity (GRAVY) |
-0.183 |
Wayengera et al. Theoretical Biology and Medical Modelling 2008 5:18 doi:10.1186/1742-4682-5-18 |
|