Research

Variance in multiplex suspension array assays: A distribution generation machine for multiplex counts

Brian P Hanley^1,2

¹Microbiology Graduate Group, University of California, Davis, CA 95616, USA

²BW Education and Forensics, 2710 Thomes Avenue, Cheyenne, Wyoming 82001, USA

author email corresponding author email

Theoretical Biology and Medical Modelling 2008, 5:3doi:10.1186/1742-4682-5-3

Published:	28 January 2008

Abstract

Background

This study attempted to replicate Luminex experimental results for large numbers of beads per classifier using multiplexed assays and routine instrument use conditions.

Conclusion

Using larger numbers of microspheres per classifier highlights a fundamental stochastic distribution of bead counts issue complicated by other factors. The more classifiers and the higher the count required per classifier there are, the more apparent the distribution of counts per classifier will be, and the more microspheres are required. Additional problems have been identified. Alternate methods of improving precision and reliability are recommended such as intraplexing and multi-well sample replicates to improve precision and confidence.