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Estimation of the solubility parameters of model plant surfaces and agrochemicals: a valuable tool for understanding plant surface interactions

Mohamed Khayet1 and Victoria Fernández2*

Author Affiliations

1 Department of Applied Physics I, Faculty of Physics, Universidad Complutense, Avda Complutense s/n, 28040, Madrid, Spain

2 Genetics and Eco-physiology Research Group, School of Forest Engineering, Technical University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain

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Theoretical Biology and Medical Modelling 2012, 9:45  doi:10.1186/1742-4682-9-45

The electronic version of this article is the complete one and can be found online at: http://www.tbiomed.com/content/9/1/45


Received:12 September 2012
Accepted:3 November 2012
Published:14 November 2012

© 2012 Khayet and Fernández; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Most aerial plant parts are covered with a hydrophobic lipid-rich cuticle, which is the interface between the plant organs and the surrounding environment. Plant surfaces may have a high degree of hydrophobicity because of the combined effects of surface chemistry and roughness. The physical and chemical complexity of the plant cuticle limits the development of models that explain its internal structure and interactions with surface-applied agrochemicals. In this article we introduce a thermodynamic method for estimating the solubilities of model plant surface constituents and relating them to the effects of agrochemicals.

Results

Following the van Krevelen and Hoftyzer method, we calculated the solubility parameters of three model plant species and eight compounds that differ in hydrophobicity and polarity. In addition, intact tissues were examined by scanning electron microscopy and the surface free energy, polarity, solubility parameter and work of adhesion of each were calculated from contact angle measurements of three liquids with different polarities. By comparing the affinities between plant surface constituents and agrochemicals derived from (a) theoretical calculations and (b) contact angle measurements we were able to distinguish the physical effect of surface roughness from the effect of the chemical nature of the epicuticular waxes. A solubility parameter model for plant surfaces is proposed on the basis of an increasing gradient from the cuticular surface towards the underlying cell wall.

Conclusions

The procedure enabled us to predict the interactions among agrochemicals, plant surfaces, and cuticular and cell wall components, and promises to be a useful tool for improving our understanding of biological surface interactions.

Keywords:
Agrochemicals; Cuticle; Plant surfaces; Solubility parameter; Waxes

Background

Plant surfaces play a major role in protection against multiple potential biotic and abiotic stress factors [1]. To adapt to these multiple functions, the plant epidermis has developed various characteristics, including specialised cell types such as trichomes or stomata [2]. Epidermal cells are surrounded by a cell wall, which plays a crucial structural and physiological role in plant development and survival [3].

Differentiation and maintenance of the epidermis are essential for plant growth and survival and require continuous cross-talk between epidermal cells and their immediate environment [2]. Epidermal cells also provide mechanical support by adhering strongly to each other via a strengthened cell wall, which is usually noticeably thicker on the external surface. In addition to the asymmetrical deposition of cell wall material, epidermal cells secrete a lipid-rich cuticle specifically into the thickened external cell wall matrix [2]. Therefore, the cuticle may be considered a cutinised cell wall, emphasizing the heterogeneous nature of this layer and its interconnection with the cell wall beneath [4]. The main protective role of the cuticle is related to the prevention of uncontrolled exchange of water and gases between the plant and the surrounding environment [5]. The functional relevance of the cuticle to plant growth and survival is evidenced by the significant commitment of epidermal cells to cuticle production [6].

The cuticle is made of a bio-polymer matrix, waxes that are deposited on to (epicuticular) or intruded into (intracuticular) this matrix, and variable amounts of polysaccharides and phenolics [4,7]. It is an asymmetric membrane [8] generally comprising three distinct layers from the outer to the inner side of the organ, namely: (i) the epicuticular wax layer, (ii) the “cuticle proper” containing waxes and cutin and/or cutan, and (iii) the “cuticular layer” composed of cutin and/or cutan and a high polysaccharide content [9].

Waxes commonly constitute 20 to 60% of the cuticle mass and are complex mixtures of straight chain aliphatics [6]. Wax composition and structure can vary among different species, organs, states of development, and environmental and stress conditions during growth [10,11]. The mechanisms of epicuticular wax formation and regeneration have been assessed in some studies [12] and it has been proposed that cuticular transpiration is the driving force behind wax movement through the cuticle [13,14].

The cuticle matrix is commonly made of cutin, which is a biopolymer formed by a network of inter-esterified, hydroxyl- and hydroxy-epoxy C16 and/or C18 fatty acids [15]. At least six different types of cuticular ultrastructures have been identified by transmission electron microscopy (TEM) [9], but their relationship to cutin monomer composition remains unclear [7,16]. The formation of cutinsomes, which are spherical nanoparticles resulting from the self-assembly of cutin hydroxyacid monomers in a polar environment, has been demonstrated; cutinsomes have been proposed as building units of the bio-polyester cutin [17].

While cutin is depolymerised and solubilised upon saponification, cuticles from some species contain a non-saponifiable and non-extractable polymer known as cutan, which yields a characteristic series of long chain n-alkenes and n-alkanes upon flash pyrolysis [18]. Cutin has been found to be the only polymer present in the cuticles of many fruits and leaves of Solanaceae and Citrus species [9], while different proportions of cutin and cutan have been determined in cuticular membranes extracted from leaves [18] and fruits such as peppers, apples or peaches [19,20].

Major differences in surface topography have been observed in different species and organs, but three hierarchical levels of structuring may occur in association with: (i) the general shape of epidermal cells, (ii) cuticular folds, and (iii) epicuticular wax crystals [21]. For example, the presence of papillae [22] or trichomes [20] can have a major effect on surface topography and wettability at the microscale level. Also, increased surface roughness and surface hydrophobicity have been reported owing to the occurrence of nano-scale structures provided by epicuticular wax crystals [22,23].

Different degrees of wettability of leaves from various species have been reported by measuring water contact angles (e.g., [21,24-26]). In addition, phyllosphere-related factors such as the deposition of aerosols or microorganisms can lead to plant surface heterogeneity [27,28], especially in urban or polluted habitats [29]. However, non-wettable surfaces have been observed to accumulate particles more slowly than wettable ones [30].

Recently Fernández et al. [20] estimated the surface free energy, polarity and work of adhesion of a model pubescent surface and proposed the implementation of membrane science approaches to exploring the physical-chemical properties of plant surfaces. It has been suggested that the cuticle acts as a “solution-diffusion” membrane for the diffusion of some solvents and solutes [31,32]. To analyse the permeability of the plant cuticle to solutes and solvents, both the solubility and diffusivity of the compounds must be taken into consideration. While diffusivity is a kinetic parameter associated with the molecular size of a compound in relation to the structure of the matrix, solubility is a thermodynamic parameter that indicates the affinity of a given chemical for the cuticle. Therefore, and as a preliminary step towards the evaluation of plant cuticle permeability, we have analysed for the first time the solubility of model plant surfaces and chemical constituents in relation to agrochemicals of commercial significance, following a thermodynamic approach. Prediction of solubility parameters is commonly used, for example, in the design and fabrication of polymeric membranes [33,34], in the coating industry [35] and also in pharmacology [36]. However, with the exception of the human skin [37,38], this procedure has not so far been applied to estimating the properties of biological surfaces.

As model plant surfaces, peach and pepper fruits were selected since they contain alkanes as major wax constituents but have significantly different surface topographies. Juvenile Eucalyptus globulus leaves, which are covered with a dense layer of nano-tubes and contain β-diketones as dominant waxes, were also evaluated for comparison.

For model plant surfaces, cuticular constituents and agrochemicals, the following hypotheses were tested: (i) is it possible to predict the solubility of plant surface constituents and the affinity of agrochemicals for plant surfaces? and (ii) can solubility parameters be used to estimate the properties of the plant cuticle?

Materials and methods

Plant material

The plant materials analysed correspond to intact, undamaged mature peaches (Prunus persica (L.) Batsch. cv. ‘Calrico’), red bell peppers (Capsicum annum L. cv. ‘Genil’) and juvenile Eucalyptus leaves (Eucalyptus globulus Labill. ssp. globulus).

Epicuticular waxes, cutin monomers and cell wall polysaccharides

The properties of the major wax constituents present in Eucalyptus leaves, bell peppers and peach fruits were used for calculating the solubility parameters (Figure 1, Table 1). Alkanes are the dominant class of compounds covering the surface of the peach fruits analysed [20]. Alkanes are also the dominant class of wax compounds extracted from pepper fruits, followed by triterpenoids such as α- or β-amyrin [39-41]. Beta-diketones are the dominant class of wax compounds in juvenile Eucalyptus leaves, but n-nonacosane, heptadecan-2-one and n-hexacosanal are also present in significant concentrations [42-46].

thumbnailFigure 1. Molecular structures of the cuticular constituents evaluated.

Table 1. Chemical formula and molar volume of the dominant epicuticular waxes extracted from Eucalyptus leaves, pepper and peach fruits and of common cutin monomers found in plant cuticles

To estimate the solubility parameter range of the cuticle matrix, calculations were carried out with model cutin monomers, which have commonly been identified in plant cuticle monomer analyses [47,48]. The selected ω-hydroxy-fatty acids are: 16-hydroxy-hexanodecanoic acid, 10,16-dihydroxy-hexanodecanoic acid, 9,10-epoxy-18-hydroxy-octadecanoic acid, and 9,10,18-trihydoxy-octadecanoic acid (Table 1). Maximal and minimal solubility parameter values were estimated per monomer according to the potential formation of ester bonds.

The solubility parameter of cellulose, a biopolymer formed from unbranched, unsubstituted (1,4)-β-D-glucan chains [3], was evaluated by estimating the properties of the D-glucose monomer. The solubility parameter range of pectins was assessed by analysing the structure of homogalacturonans based on α-1-4 linked, D-galacturonic acid Table 2; [3,49].

Table 2. Characteristics of the chemicals used for estimation of solubility parameters

Chemicals

Several compounds with different properties and degrees of complexity were selected for calculation of solubility parameters (Figure 2, Table 2). The densities of urea and sorbitol were obtained from the PubChem Bioassay Database (http://pubchem.ncbi.nlm.nih.gov webcite; identification codes: 1176, 5780 and 311, respectively). The molecular structures of plant protection active ingredients (flutolanil, fenoxycarb, chlorothalonil, formetanate, esfenvalerate and cypermethrin) were obtained from ChemSpider with some modifications (Royal Society of Chemistry, UK). Details of the densities of flutolanil, fenoxycarb and fometanate were collected from the Pesticide Properties Database (University of Hertforshire, UK). The densities of chlorothalonil, esfenvalerate and cypermethirn were derived from the European Union Pesticides Database, while data on Genapol X-80 (8 ethylene oxide (EO) units, 13.4 hydrophile-to-lipophile balance (HLB)) and Triton X-100 (assuming 10 EO units and 14.1 HLB) were obtained from Sigma-Aldrich product data-sheets. The density of Brij 35 (23 EO units, 17.1 HLB) was obtained from ChemSpider. Molecular weights were calculated from the number of atoms, and molar volumes were estimated by dividing the molecular weight by the density (Table 2).

thumbnailFigure 2. Molecular structures of the agrochemicals selected for calculation of solubility parameters.

Microscopy

Gold-sputtered intact Eucalyptus adaxial leaves, peach and pepper fruit surfaces were examined with a Hitachi S-3400 N (Tokyo, Japan) and a Philips XL30 (Eindhoven, The Netherlands) scanning electron microscope (SEM).

For TEM observations of Eucalyptus leaf tissue, approximately 1 mm2 sections were cut with a scalpel and fixed in 2% paraformaldehyde plus 2% glutaraldehyde (both from Electron Microscopy Sciences (EMS), Hatfield, USA) for 6 h in ice-cold phosphate buffer (pH 7.2). Samples were subsequently washed five times in phosphate buffer, kept at 4°C overnight, and post-fixed with 1% osmium tetraoxide (TAAB Laboratories, Berkshire, UK) and 1.5% potassium ferrocyanide (Sigma-Aldrich, Munich, Germany) in distilled water (2 h). They were then rinsed in distilled water (3 × 10 min) and dehydrated in an acetone:water (v/v) series of 50, 60, 70, 80, 90, 95 (2 × 10 min each) and 100% (3 × 10 min). The tissues were successively immersed in 1:3 (2 h), 1:1 (2 h) and 1:3 (3 h) Spurr’s resin:acetone (v/v) solutions and kept overnight in pure Spurr’s resin (TAAB Laboratories). The samples were finally placed in moulds and were incubated at 60°C for three days. Ultrathin sections were stained with uranyl acetate (20 min) and lead citrate (4 min; both chemicals from EMS) and were examined by TEM (Jeol JEM-1010, Tokyo, Japan).

Contact angle measurements and prediction of solubility parameters

Advancing contact angles of drops of double-distilled water, glycerol and diiodomethane (both 99% purity, Sigma-Aldrich) were measured at 25°C using a CAM 200 contact angle meter (KSV Instruments Ltd., Helsinki, Finland). Contact angles were measured on intact Eucalyptus adaxial leaf, peach and pepper fruit surfaces (30 repetitions). The plant surfaces analysed were collected from fruits and leaves previously observed by SEM. No materials that could affect contact angle measurements (e.g., salt deposits or microorganisms) were found to be deposited on them.

Two μL drops of each liquid were deposited on to the plant surfaces with a manual dosing system holding a 3 mL syringe (0.5 mm diameter needle). Side view images of the drops were captured at a rate of 10 frames s-1. Contact angles were automatically calculated by fitting the captured drop shape to the one calculated from the Young–Laplace equation.

For the three plant surfaces evaluated, the total surface free energy, including its three components (i.e. the Lifshitz-van der Waals (LW), acid (+) and base (−) components), was calculated in addition to the surface polarity and work of adhesion [20].

Prediction of solubility parameters

The solubility parameter of each plant surface analysed, δθ, was calculated from the following equation [50]:

<a onClick="popup('http://www.tbiomed.com/content/9/1/45/mathml/M1','MathML',630,470);return false;" target="_blank" href="http://www.tbiomed.com/content/9/1/45/mathml/M1">View MathML</a>

(1)

where ec (MJ m-3) is the cohesive energy density, which is related to the surface free energy, γs, (mJ m-2) as follows:

<a onClick="popup('http://www.tbiomed.com/content/9/1/45/mathml/M2','MathML',630,470);return false;" target="_blank" href="http://www.tbiomed.com/content/9/1/45/mathml/M2">View MathML</a>

(2)

The solubility parameter of a material can be calculated from either the cohesive energy (Eqn. 1) or the molar attraction constant, F ((MJ/m3)1/2 mol-1), as:

<a onClick="popup('http://www.tbiomed.com/content/9/1/45/mathml/M3','MathML',630,470);return false;" target="_blank" href="http://www.tbiomed.com/content/9/1/45/mathml/M3">View MathML</a>

(3)

where v is the molar volume (cm3 mol-1) of the molecule [51].

The solubility parameter has three components taking into account the interactions due to dispersion forces (δd), polar forces (δp) and hydrogen (H)-bonding (δh), and it is expressed as:

<a onClick="popup('http://www.tbiomed.com/content/9/1/45/mathml/M4','MathML',630,470);return false;" target="_blank" href="http://www.tbiomed.com/content/9/1/45/mathml/M4">View MathML</a>

(4)

According to van Krevelen and Hoftyzer [52], the solubility parameter components can be predicted from group contributions, using the following equations:

<a onClick="popup('http://www.tbiomed.com/content/9/1/45/mathml/M5','MathML',630,470);return false;" target="_blank" href="http://www.tbiomed.com/content/9/1/45/mathml/M5">View MathML</a>

(5)

<a onClick="popup('http://www.tbiomed.com/content/9/1/45/mathml/M6','MathML',630,470);return false;" target="_blank" href="http://www.tbiomed.com/content/9/1/45/mathml/M6">View MathML</a>

(6)

<a onClick="popup('http://www.tbiomed.com/content/9/1/45/mathml/M7','MathML',630,470);return false;" target="_blank" href="http://www.tbiomed.com/content/9/1/45/mathml/M7">View MathML</a>

(7)

where Fdi and Fpi are the molar attraction constants of the dispersion and polar components, respectively, Ehi is the H-bonding energy and v is the molar volume.

The contributions of the functional groups present in the chemicals and plant structural compounds analysed to the solubility parameter components are shown in Table 3. From the solubility parameter components, the total solubility parameter (δ) can be calculated from Equation 4, and is hereafter named δm for agrochemicals, δwax for epicuticular waxes and δnm for cutin and polysaccharide monomers.

Table 3. Contributions of structural groups present in the selected molecules to the solubility parameter component[52]

Finally, to evaluate the affinity of a polymer for a solvent [51] or the affinity of an agrochemical for a given plant surface, the following equation was used:

<a onClick="popup('http://www.tbiomed.com/content/9/1/45/mathml/M10','MathML',630,470);return false;" target="_blank" href="http://www.tbiomed.com/content/9/1/45/mathml/M10">View MathML</a>

(8)

Moreover, to study the affinities of the agrochemicals for plant surfaces as derived from the solubility parameter calculated from contact angle measurements (δθ), the following equation was applied:

<a onClick="popup('http://www.tbiomed.com/content/9/1/45/mathml/M11','MathML',630,470);return false;" target="_blank" href="http://www.tbiomed.com/content/9/1/45/mathml/M11">View MathML</a>

(9)

The results from Equations 8 and 9 imply that the lower the values of Δδwax and Δδθ, the higher the affinity between agrochemical and plant surface.

Results

Surface topography and hydrophobicity

The contact angles (in °) of water, glycerol and diiodomethane with plant surfaces are (mean ± standard deviation): 142.6 ± 6.7, 136.5 ± 11.2 and 84.0 ± 7.0 for Eucalyptus leaves; 83.4 ± 4.7, 68.6 ± 9.2 and 60.8 ± 6.2 for pepper fruits; and 134.2 ± 7.0, 130.9 ± 7.0 and 55.7 ± 3.9 for peach fruits (see Figure 3 as an illustration of the measurements).

thumbnailFigure 3. Contact angle measurements on intact, adaxial Eucalyptus (A,C,E) leaf surfaces and pepper fruit surfaces (B,D,F). Drops of: (A,B) water, (C,D) glycerol, and (E,F) diiodomethane.

The topographies of the plant materials analysed are shown in Figure 4. The adaxial surface of juvenile Eucalyptus leaves is densely covered with a network of wax nano-tubes, which can be clearly identified as such at higher magnifications (Figure 4D and G). In contrast, the pepper fruit surface is covered with a pattern of epidermal cells (Figure 4B and E) and epicuticular waxes with no clear structure, yielding a smooth and rather flat surface. The peach surface is densely covered with conspicuous trichomes (approximately 1 mm long and 20 μm thick), which provide a high degree of micro-scale roughness (Figure 4C, F and I) in contrast to the nano-scale surface roughness of Eucalyptus leaves (Figure 4G). Given the dense and thick network of micro- (trichomes) and nano- (epicuticular waxes) tubes covering peach fruits and Eucalyptus leaves, respectively, we could not determine their roughness by atomic force microscopy.

thumbnailFigure 4. Scanning electron micrographs of intact plant surfaces.Eucalyptus adaxial leaf surfaces: (A) ×400, (D) ×1,000, and (G) ×9,000. Pepper fruit surfaces: (B) ×200, (E) ×400, and (H) ×1,000. Peach fruit surfaces: (C) ×100, (F) ×500, and (I) ×1,300.

Adaxial Eucalyptus leaf surfaces are almost super-hydrophobic and the peach fruit surface is also very hydrophobic, while the pepper fruit surface is more wettable.

Surface free energy, polarity, work of adhesion and solubility parameter

Measurement of contact angles with water, glycerol and diiodomethane enabled several plant surface properties to be calculated (Table 4). The total surface free energies of peach and pepper fruits are similar and significantly higher than that measured for Eucalyptus leaves (approximately 32.2 versus 17.4 mJ m-2, respectively). In all cases, there is a major contribution of the Lifshitz-van der Waals component, while the acid–base component is more significant in pepper fruits. Peach and Eucalyptus surfaces have higher contributions from electron acceptor interactions, whilst electron donor interactions predominate in pepper. The lowest and the highest surface polarities correspond to peach and pepper fruits, respectively. The work of adhesion for water and glycerol is much higher in pepper fruits (81.2 mJ m-2) than peach fruits and Eucalyptus leaves (between 15.0 and 22.1 mJ m-2; Table 4). However, the work of adhesion for diiodomethane lies within a similar range to that for peach and pepper fruits, and is significantly lower in Eucalyptus leaves.

Table 4. Surface free energy per unit area, Lifshitz van der Waals component LW), acid–base component (γAB) with the contributions of electron donor (γ-) and electron acceptor +) interactions, total surface free energy ( γ ), surface polarity AB γ -1), solubility parameter (δθ) and work of adhesion (for water, Ww; glycerol, Wg; diiodomethane, Wd) of adaxial Eucalyptus leaf, pepper and peach fruit surfaces

Concerning the solubility parameters (δθ) of the three plant materials analysed (Table 4), Eucalyptus leaves exhibit a significantly lower value (10.6 MJ1/2 m-3/2) than pepper and peach fruit surfaces (approximately 17 MJ1/2 m-3/2).

Solubility parameter of epicuticular waxes

The solubility parameters of the most abundant epicuticular waxes (δwax) of Eucalyptus leaves, pepper and peach fruits are shown in Table 5.

Table 5. Total solubility parameter (δwax) and solubility parameter components of the most abundant epicuticular waxes of Eucalyptus leaves, pepper and peach fruits

The dominant class of compounds in both pepper and peach fruit waxes is n-alkanes, which have a δwax around 16 MJ1/2 m-3/2 for the most abundant compounds reported (C23 to C31n-alkanes). However, it is remarkable that such compounds lack polar (δp) and H-bonding (δh) solubility parameter components. While n-alkanes are the most abundant waxes in peach, a relatively abundant class of triterpenoids (e.g., α,β-amyrin) can be found in pepper fruits. Although the presence of such waxes will not significantly modify δwax (16.7 MJ1/2 m-3/2, for α,β-amyrin), they could potentially facilitate interactions due to polar forces and H-bonding owing to the presence of a functional alcohol group.

With regard to Eucalyptus leaves, the dominant class of compounds is β-diketones, which have a δwax of approximately 16.6 MJ1/2 m-3/2 and non-zero δp and δh components owing to the presence of ketone functional groups. Other wax classes often found in Eucalyptus leaves are n-alkanes (chiefly n-nonacosane), alkanals (aldehydes) such as hexacosanal, and ketones (e.g., heptadecan-2-one). These kinds of waxes and some others not included in Table 2 (data not shown) were found to have δwax values ranging between 16 and 17 MJ1/2 m-3/2, but in contrast to n-alkanes they all have contributions from δp and δh because of the presence of aldehyde, ketone and/or alcohol functional groups.

For peach and pepper fruits, the values of δθ and δwax are within the same range (16 to 17 MJ1/2 m-3/2). However, a significantly lower δθ value (10.6 MJ1/2 m-3/2) was determined for Eucalyptus leaf surfaces than the δwax calculated for β-diketones (approximately 16.6 MJ1/2 m-3/2). This may be attributed to the nano-structure of the Eucalyptus leaf surface, which decreases the δθ value in association with a high degree of surface roughness and hydrophobicity, as shown for various synthetic and natural materials [53].

Solubility parameters of agrochemicals

The total solubility parameters (δm) and solubility parameter components of the selected molecules are shown in Table 6. The water-soluble compounds urea and sorbitol have high δm values and major contributions from δp and especially δh. A similarly high δm value was determined only for the non-systemic fungicide chlorothalonil, which also has the highest δd value of all the compounds considered.

Table 6. Total solubility parameters (δm) and solubility parameter components of agrochemicals

Insecticides (esfenvalerate, fenoxycarb, α-cypermethrin and formetanate) and the fungicide flutolanil have δm values ranging between 20 to 24 MJ1/2 m-3/2, with a major contribution from the δd component. In contrast, a higher δm was determined for the fungicide chlorothalonil. The results also indicate that compounds with different chemical structures such as flutolanil, fenoxycarb and esfenvarelate can have similar δm values.

The δm values of the three non-ionic surfactants are between 19.5 and 21.4 MJ1/2 m-3/2 and the differences among them are chiefly associated with the values of the δd and δp components. The major difference between Genapol X-80 and Brij 35 is related to δp (8.1 and 5.9 MJ1/2 m-3/2, respectively). The alkyl-phenol surfactant Triton X-100 lies between the values calculated for the two alkyl ethoxylates (δm = 20.2 MJ1/2 m-3/2).

Affinity of agrochemicals for plant surfaces

Results concerning the affinity of agrochemicals for plant surfaces in relation to δθ and δwax are shown in Figure 5. The affinities of chemicals for the dominant epicuticular waxes present in Eucalyptus leaves, pepper and peach fruits are within a similar range (Figure 5A, B and C, light grey bars). The n-alkanes present in pepper and peach fruit surfaces provide a lower affinity for agrochemicals than the β-diketones of the Eucalyptus leaf.

thumbnailFigure 5. Affinity of agrochemicals for Eucalyptus leaf (A), pepper (B) and peach fruit (C) surfaces. Light grey bars represent the calculated Δδwax, based on the solubility of agrochemicals in relation to the most abundant epicuticular wax compound (δwax), and dark grey bars refer to Δδθ(± standard errors), calculated from contact angle measurements (δθ).

The compounds with the lowest affinity for the epicuticular waxes covering the three plant materials analysed (i.e., those with the highest Δδwax values) are urea and sorbitol. Regarding the plant protection active ingredients, the highest affinity for epicuticular waxes was calculated for esfenvalerate and flutolanil, followed by fenoxycarb and formetanate. The compounds α-cypermethrin and chlorothalonil have higher Δδwax values and hence a lower affinity for the dominant waxes. Genapol X-80 is the surfactant with the highest affinity for epicuticular waxes, followed by Triton X-100.

The range of affinities of the agrochemicals for pepper and peach fruit surfaces based on contact angle measurements (Δ δθ) is similar to the range predicted from the dominant epicuticular waxes (Δδwax). In contrast, lower affinities of agrochemicals for the Eucalyptus leaf surface were estimated in relation to contact angle measurements, which take into account the combined effects of surface chemistry and roughness (Figure 5, dark grey bars).

Solubility parameter gradient across the plant surface

The total solubility parameters (δmn) and solubility parameter components of model cutin monomers and cell wall polysaccharides are shown in Table 7. The values estimated for free ω-hydroxy-fatty acids, D-glucose and D-galacturonic acid are higher than those calculated after monomer esterification or formation of glycosidic bonds, mainly because the H-bonding component is lower.

Table 7. Total solubility parameters (δmn) and solubility parameter components of common cutin monomers and cell wall constituents

The δmn values of the monomers were estimated by determining the possible minimum and maximum values in association with the potential occurrence of more than two ester bonds (e.g., on 9,10,18-tryhidroxy-octadecanoic acid). The presence of free hydroxyl groups will raise the value of δh and hence increase the total solubility parameter. This can be observed, for example, by contrasting 10,16-oxy-hexanodecanoate (three ester bonds) with 10-oxy, 16-hydroxy-hexanodecanoate (two ester bonds); the δmn values are 17.9 and 19.4 MJ1/2 m-3/2, respectively. Similarly, the monomer 9,12,18-oxy-octadecanoate (four ester bonds; 17.7 MJ1/2 m-3/2) has a lower δmn value than 9-oxy, 18,12-dihydroxy-octadecanoate (two ester bonds; 20.0 MJ1/2 m-3/2). The cutin monomers derived from 16-hydroxy-hexanodecanoic and 9,10-epoxy-18-hydroxy-octadecanoic acid can only form two ester bonds and have the lowest δmn parameter.

The total solubility parameters determined for polymerised D-glucose and D-galacturonic acid (around 32.2 MJ1/2 m-3/2) are remarkably above the range assessed for waxes and cutin, chiefly because of the major contributions of the δp and δh components. Methylation of the carboxylic group in the pectic compound leads to only a slight increase of the δmn parameter (data not shown).

By analysing the solubility parameter results estimated for the different materials covering epidermal cells and for the dominant epicuticular waxes present in the three species used for contact angle determinations, a solubility parameter gradient from the outer to the inner side of the cuticle can be expected as depicted in Figure 6. The epicuticular wax layer, which is in direct contact with the atmosphere, has the lowest solubility parameter value and may often lack polar and H-bonding components, as observed for n-alkanes. Cutin monomers usually form the cuticular matrix and their degree of polymerization can alter the total solubility parameter of the biopolymer (ranging between 17 and 20 MJ1/2 m-3/2). In the more external cuticular layers there are variable proportions of waxes and cutin (i.e., in the epicuticular wax layer and cuticle proper). Polysaccharides are present in the cuticular layer in direct contact with the cell wall. Therefore, according to current views on the composition of the materials (cuticle and cell wall) covering epidermal plant cells, a solubility parameter gradient is established from the external and more hydrophobic epicuticular wax layer towards the more hydrophilic internal cell wall.

thumbnailFigure 6. Solubility parameter gradient model for the cuticle and cell wall covering the epidermis of plants. The three cuticular layers are indicated as: EWL (epicuticular wax layer), CP (cuticle proper) and CL (cuticular layer). From the EWL (−) to the cell wall (+) there is a gradual solubility parameter increase. The TEM micrograph corresponds to a transverse section of a juvenile, adaxial Eucalyptus globulus leaf surface (×80,000).

Discussion

In this study, a procedure for predicting the interactions among different structural plant surface constituents and agrochemicals, based on the estimation of solubility parameters, has been introduced for the first time in a plant science context. While prediction of solubility parameters is commonly used in membrane science [33] and also in pharmacology [36], this procedure has not so far been applied to estimating the surface properties of biological materials, with the exception of a few studies focused on human skin [37,38].

The permeability of a compound through a plant cuticle is the product of its solubility, which is a thermodynamic parameter reflecting the degree of interaction between that compound and the plant cuticle, and its diffusivity through the matrix of the plant cuticle, which is a kinetic parameter associated with the molecular size of the compound and the structure of the matrix. This study is only focused on analysing for the first time the solubilities (not the permeabilities) of model plant surfaces and chemical constituents in relation to agrochemicals of commercial significance, adopting a thermodynamic perspective.

Two different approaches have been followed to assess the solubility parameters of plant surfaces. One is based on contact angle measurements (δθ), which reflect both physical and chemical effects associated with the topography and composition of the surface. The other is limited to considering the nature of the dominant epicuticular waxes covering the surface (δwax). The three materials selected are good examples of the diversity of plant surface structures in relation to the variability of cell shapes and micro- and nano-structures on the cell surfaces. In addition, the similar chemical composition of the dominant waxes in pepper and peach fruits in contrast to the β-diketones prevailing in juvenile Eucalyptus leaves offers another interesting aspect to evaluate in the plant surfaces investigated. From an agrochemical viewpoint, substances with different activities, polarities, and degrees of complexity were assessed as model compounds.

Plant surface properties

The adaxial Eucalyptus leaf surface is almost superhydrophobic and has a high degree of nano-roughness conferred by the dense network of wax nano-tubes [54]. However, the very hydrophobic surface of the highly pubescent peach variety analysed has a high degree of micro-roughness provided by the trichome network. In contrast, the pepper fruit surface has a smooth topography and it is more wettable than the other plant surface samples.

The determination of contact angles of three liquids enabled the three distinct plant materials to be compared by a novel approach [20]. Consequently, the three plant surfaces were characterised in terms of surface free energy, polarity, work of adhesion and solubility parameter. Fruit surfaces have the highest surface free energies and solubility parameter values, in contrast to the Eucalyptus leaf surface. However, the peach skin is the surface with the lowest polarity, which implies the lowest degree of potential polar and H-bonding interactions among the three surfaces analysed. The pepper surface has a significantly higher work of adhesion for water than the peach fruit and the Eucalyptus leaf surface. This indicates that water drops falling on the pepper fruit surface will be retained, in contrast to the repulsion of the drops falling on to peach fruit and especially Eucalyptus leaf surfaces. Therefore, the behaviour of the plant surfaces evaluated may bring some ecophysiological advantage to the plant organs and related species, which future studies should investigate further [20].

Furthermore, the proposed tools based on contact angle measurements of the three liquids may be useful for investigating plant surface dynamics during the growing season or as affected by plant biotic or abiotic stress factors. For example, epicuticular wax erosion in association with environmental pollution [29] or the deposition of aerosols and microorganisms [27,28] could increase the degree of heterogeneity and wettability of the surface, ultimately affecting plant-water relationships [55].

Solubility parameters of cuticular components and agrochemicals

The solubility parameters of the dominant waxes in the analysed surfaces lie within the range 16.0 to 16.7 MJ1/2 m-3/2. Such a range is representative of a wide number of wax compounds such as β-diketones, n-alkanes, amyrins, and a few other additional compounds having aldehyde, ketone, and alcohol functional groups. The model cutin monomers evaluated have higher solubility parameters than waxes, varying between 19 and 22 MJ1/2 m-3/2 for the free ω-hydroxy-fatty-acids and possibly falling to 17 MJ1/2 m-3/2 after esterification of all functional hydroxyl groups. The model cellulose and pectin monomers have considerably higher solubility parameters than other cuticle constituents, which supports previous observations on the hydrophobicity of the cuticle as compared to the cell wall [2].

The presence of wax compounds such as n-alkanes renders the plant surface apolar. Despite the smooth topography and wettability of the pepper fruit, its n-alkane coating will only lead to the occurrence of dispersive interactions with surface-deposited materials and liquids, a phenomenon that can also be expected for peach fruits.

The different numbers of EO units in the surfactants evaluated had only a slight effect on the total solubility parameter. A correlation between surfactant solubility parameters and HLB or critical micelle concentrations has been reported [56,57]. The surfactant with the lowest solubility parameter (Genapol X-80) was recorded as having the lowest surface tension (approximately 27 mJ m-2 at 0.1%) while the highest surface tension (around 45 mJ m-2 at 0.1%) and solubility parameter were estimated for Brij 35.

It must be noted that water has a total solubility parameter of 47.9 MJ1/2 m-3/2[58] and that all chemicals except urea and sorbitol, which can be supplied at concentrations above 1%, were applied at approximately 0.1% concentrations. However, it can be expected that the chemicals assessed will interact with epicuticular waxes once sprayed on to plant surfaces as aqueous solutions, especially if surface-active agents are applied to improve contact between the solid and the liquid.

Affinities of agrochemicals for plant surfaces

The affinities of agrochemicals for plant surfaces were evaluated on the basis of contact angle measurements and by considering the chemical structures of the dominant epicuticular waxes. According to Greenhalgh et al. [59], compounds with a solubility parameter difference (Δδ) below 7 MJ1/2 m-3/2 are likely to be miscible, while chemicals with a Δδ higher than 10 MJ1/2 m-3/2 are likely to be immiscible. The agrochemicals and plant surfaces selected in this study were found to have Δδ values ranging between 2.5 MJ1/2 m-3/2 (the highest affinity) and 26.6 MJ1/2 m-3/2 (no affinity). This indicates that such plant surfaces have a high affinity for some agrochemicals (Genapol X-80, flutolanil, esfenvalerate, Triton X-100, fenoxycarb, Brij 35, formetanate), which can readily penetrate into the plant organ (leaf or fruit in this case), and less affinity for other compounds (α-cypermethrin, sorbitol, urea and chlorothalonil).

The plant protective chemicals with the highest affinities for plant surfaces were found to be those with the lowest total solubility parameters. The high affinities of esfenvalerate, flutolanil and fenoxycarb for the surfaces evaluated make these compounds more prone to cuticular uptake and sorption into plant tissues. Since such plant protection products can be sprayed on to the leaves and fruits of agro-forest species, the estimation of solubility parameters could be used as a complementary tool for pesticide risk assessment. Therefore, the compounds with the lowest toxicity risk will be those with lower affinities for plant surfaces. The proposed methodology may be useful for improving the performance of foliar sprays of e.g., plant protection products, herbicides and fertilisers, taking into account their mode of action (e.g., systemic or contact) and the surface properties of the target organism (e.g., the plant, or surface pathogens and pests).

The surfactants selected in this study also have high affinities for plant surfaces, especially in the case of Genapol X-80. Surfactant solutions sprayed on to foliage have often been observed to be phytotoxic [60], which may be associated with the high solubility of some of these compounds in plant surfaces.

The lower affinities of agrochemicals for the Eucalyptus leaf estimated from contact angle measurements are due to the major roughness provided by the wax nano-tubes that densely cover the surface. These nano-tubes are also responsible for the high degree of hydrophobicity of the material [22,23]. In contrast, such differences between the affinities predicted from contact angle measurements and epicuticular wax chemistry were not observed for the peach and pepper fruit surfaces. The micro-scale roughness provided by the dense layer of trichomes covering the peach surface markedly increases the hydrophobicity of that surface, but seems to have limited effect on the solubility parameter.

Solubility parameter gradient model

To gain insight into the characteristics of the cuticle and the cell wall by calculating solubility parameters, additional estimations were made for common cuticular matrix constituents and cell wall polysaccharides. To our knowledge, this is the first time in which the polar, dispersive and H-bonding properties of plant cuticular and cell wall constituents have been interpreted in quantitative terms. Owing to the properties of the dominant epicuticular waxes present in the three analysed plant materials, it is concluded that the solubility parameter increases with increasing depth from the epicuticular wax surface towards the cell wall. The solubility parameters determined for the model cellulose and pectin compounds are much higher than those for cutin monomers and epicuticular waxes but are still far away from the value of water.

Given the ubiquitous presence of waxes, cutin and polysaccharides in the layers covering plants’ epidermal cells [48], and assuming that the chemical constituents selected are representative of a wide range of species with hydrophobic surface properties and within the same range of potential alternative chemical components, a solubility parameter gradient is observed for a model plant surface, which can be applied to e.g., adaxial and abaxial leaf, fruit, flower or trichome surfaces that are covered with a cuticle. On the basis of thermodynamic principles, compounds with a low surface free energy (i.e., a low solubility parameter) will tend to migrate from the plant cell wall towards the epicuticular wax layer in order to decrease the Gibbs free energy [50]. This could be an alternative and/or complementary hypothesis to explain the migration of cuticular material (waxes and cutin) towards the air/plant interface, in contrast to cuticular transpiration as a driving force [13,14,17].

As noted by Scherbatskoy & Tyree [61], cuticular polymers contain polar and ionisable substituents, providing the cuticle with polar hydrophilic regions and ion exchange capacity. The topography and chemistry of epicuticular waxes generally provide a lower solubility parameter than the one prevailing in the cuticle proper and principally in the cuticular layer, where significant amounts of polysaccharides are present. The lack of polar and H-bonding functional groups in the dominant epicuticular waxes and a higher degree of monomer esterification in the cuticle matrix will tend to lower the total solubility parameter of the membrane by reducing the polar and H-bonding components. The presence of cutan can also decrease the solubility parameter of the cuticle matrix to some extent. According to Jeffree [9], cutanization (i.e., the gradual formation of cutan in the cuticle matrix) of the cuticular layer, as reported for the Clivia minata leaf cuticle [62], can arise from a maturation process involving the progressive modification of the previously deposited cutin and any embedded polysaccharides and waxes. The author suggests that the progressive reduction in reactivity of all components of the cuticular layer during cutanisation indicates that all types of polar functional groups are systematically eliminated during this maturation phase.

In order to calculate the solubility parameters, the properties of the trichomes covering the peach skin surface were considered. In this case the cuticular matrix is composed exclusively of cutin, in contrast to the high percentage of cutan in shaved cuticular membranes [20]. According to the cutan hypothesis proposed by Jeffree [9], the cuticular matrix of the peach fruit has a lower solubility parameter than cutin as the dominant cuticular matrix bio-polymer. However, the cuticular domain of the peach fruit cuticle will also have contributions from cutin and polysaccharides, which will gradually raise the total solubility parameter of the membrane as it comes closer to the cell wall. The cuticular matrix of pepper is mainly made of cutin [41], but an insoluble fraction likely to be cutan has also been identified [19]. No information is currently available on the composition of the Eucalyptus globulus leaf cuticle matrix, but the major reduction in solubility parameter associated with the nano-scale roughness of the Eucalyptus surface supports the occurrence of the solubility parameter gradient shown in Figure 6.

While most aerial plant surfaces are believed to be covered with a cuticle based on a cutin and/or cutan matrix, which contains variable amounts of waxes and polysaccharides, trials with more hydrophilic surfaces and different materials should be carried out in the future to estimate the solubility parameters of plant surface chemical constituents quantitatively.

Conclusions

A novel method for predicting the interactions between plant surface structural constituents, plant surfaces and agrochemicals has been introduced, which was useful for predicting the solubilities of plant surface constituents and the affinities of agrochemicals for plant surfaces. Calculation of the solubility parameters of plant surface constituents led us to observe a solubility parameter gradient established from the cuticular surface towards the wall covering epidermal cells. Comparison of solubility parameters between cuticular and cell wall components will be helpful for clarifying the structure and development of the cuticle from an ontological viewpoint and also for establishing a relationship between the chemical composition and structure of the cuticular membrane, which is currently lacking. The methodology should also be of interest for multiple biological purposes and could help us understand surface phenomena on multiple biological materials.

Abbreviations

EO: Ethylene oxide; HLB: Hydrophile-to-lipophile balance; SEM: Scanning Electron Microscopy; TEM: Transmission Electron Microscopy.

Competing interests

The authors declare that they do not have any competing interests.

Authors' contributions

MK had the preliminary idea of calculating solubility parameters for plant surfaces and agrochemicals both theoretically and in relation to contact angle measurements. VF selected the model chemicals, plant surfaces and plant surface constituents and carried out the experiments. Dynamic discussions between the two authors enabled solubility parameters and affinity between chemicals to be calculated and a solubility parameter model for the plant surface to be developed. Both authors contributed to writing and improving the paper and approved the final manuscript.

Acknowledgements

The authors wish to thank Dr. M. Steinbauer (La Trobe University, Victoria, Australia), Dr. M.A. Jenks (Western Regional USDA-ARS Biomass Research Center, USA) and E.P. Parsons (Purdue University, USA) for providing information on the composition of epicuticular waxes. We would like to thank the Electron Microscopy National Center (UCM; Madrid, Spain) for technical assistance with TEM. Victoria Fernández is supported by a “Ramón y Cajal” contract (MICINN, Spain), co-financed by the European Social Fund.

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